Fluorescence lifetime image scanning microscopy (Conference Presentation)

Ingo Gregor; Niels Radmacher; Jörg Enderlein

doi:10.1117/12.2546532

9 March 2020 Fluorescence lifetime image scanning microscopy (Conference Presentation)

Ingo Gregor, Niels Radmacher, Jörg Enderlein

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 1124606 (2020) https://doi.org/10.1117/12.2546532
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Image Scanning Microscopy (ISM) enhances the spatial resolution of a confocal microscope by simple means. An extension to realize fluorescence lifetime imaging in combination with ISM seems straight-forward. First realizations have been reported by Israel et al. and Castello et al. [1,2] Here, we present a cost-efficient detector scheme based on a commercial multi-anode PMT that allows to perform fluorescence lifetime imaging microscopy (FLIM) in combination with ISM. We developa dedicated amplification electronics that allows for counting signals from 32 detector pixels using a commercial eight channel TCSPC hardware. [1] Y. Israel, R. Tenne, D. Oron, and Y. Silberberg “Quantum correlation enhanced super-resolution localization microscopy enabled by a fibre bundle camera” Nat. Commun. 8 (2017) 14786. [2] M. Castello et al. “A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM” Nat. Methods 16 (2019) 175-178.

Conference Presentation

Citation Download Citation

Ingo Gregor, Niels Radmacher, and Jörg Enderlein "Fluorescence lifetime image scanning microscopy (Conference Presentation)", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124606 (9 March 2020); https://doi.org/10.1117/12.2546532

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available