Mesoscale imaging of neuronal activity coupled with light-evoked motor mapping reveal movement-specific spatiotemporal patterns of cortical activation in awake mice

Francesco Resta; Anna Letizia Allegra Mascaro; Elena Montagni; Giuseppe de Vito; Alessandro Scaglione; Francesco Saverio Pavone

doi:10.1117/12.2544214

21 February 2020 Mesoscale imaging of neuronal activity coupled with light-evoked motor mapping reveal movement-specific spatiotemporal patterns of cortical activation in awake mice

Francesco Resta, Anna Letizia Allegra Mascaro, Elena Montagni, Giuseppe de Vito, Alessandro Scaglione, Francesco Saverio Pavone

Author Affiliations +

Proceedings Volume 11226, Neural Imaging and Sensing 2020; 112260I (2020) https://doi.org/10.1117/12.2544214
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We developed an all-optical system that couples large-scale cortical imaging with chronic light-based motor mapping in awake mice. By AAV-mediated cortical transfection, we induced the co-expression of the red-shifted genetically encoded calcium indicator and a light-sensitive optogenetic actuator ChR2 over both the rostral and caudal forelimb areas, which was stable over several months. No evidence of cross-talk was detected during illumination of ChR2+ neurons with the light source used for RCaMP1a excitation. Light-based motor mapping coupled with wide-field imaging of neuronal activation in awake mice revealed spatiotemporal patterns of cortical activation specific for movement category.

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Francesco Resta, Anna Letizia Allegra Mascaro, Elena Montagni, Giuseppe de Vito, Alessandro Scaglione, and Francesco Saverio Pavone "Mesoscale imaging of neuronal activity coupled with light-evoked motor mapping reveal movement-specific spatiotemporal patterns of cortical activation in awake mice", Proc. SPIE 11226, Neural Imaging and Sensing 2020, 112260I (21 February 2020); https://doi.org/10.1117/12.2544214

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