5-ALA induced PpIX fluorescence guided surgery: a clinical study of spectral complexity in healthy tissues and margin boundaries in high and low grade gliomas

L. Alston; L. Mahieu-Williame; M. Hebert; P. Kantapareddy; D. Meyronet; D. Rousseau; J. Guyotat; B. Montcel

doi:10.1117/12.2526950

19 July 2019 5-ALA induced PpIX fluorescence guided surgery: a clinical study of spectral complexity in healthy tissues and margin boundaries in high and low grade gliomas

L. Alston, L. Mahieu-Williame, M. Hebert, P. Kantapareddy, D. Meyronet, D. Rousseau, J. Guyotat, B. Montcel

Author Affiliations +

Proceedings Volume 11073, Clinical and Preclinical Optical Diagnostics II; 110730T (2019) https://doi.org/10.1117/12.2526950
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Gliomas are diffuse brain tumors still hardly curable due to the difficulties to identify their margins. 5-ALA induced PpIX fluorescence measurements have enabled to gain in sensitivity for discriminating margin from healthy tissue but they remain limited. In this study, we assume that two states of PpIX contribute to total fluorescence. We show that fluorescence in low density margins of high grade gliomas or in low grade gliomas comes mainly from PpIX peak centered at 620 nm. These results could help to improve fluorescence-guided resection of gliomas by discriminating healthy tissues from tumor margins.

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L. Alston, L. Mahieu-Williame, M. Hebert, P. Kantapareddy, D. Meyronet, D. Rousseau, J. Guyotat, and B. Montcel "5-ALA induced PpIX fluorescence guided surgery: a clinical study of spectral complexity in healthy tissues and margin boundaries in high and low grade gliomas", Proc. SPIE 11073, Clinical and Preclinical Optical Diagnostics II, 110730T (19 July 2019); https://doi.org/10.1117/12.2526950

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