Imaging of Aβ-deposits in ex-vivo Alzheimer's disease brain tissue using Raman spectroscopy (Conference Presentation)

Benjamin Lochocki; Tjado H. J. Morrema; Jurre den Haan; Freek Ariese; Femke H. Bouwman; Jeroen J. Hoozemans; Johannes F. de Boer

doi:10.1117/12.2509297

4 March 2019 Imaging of Aβ-deposits in ex-vivo Alzheimer's disease brain tissue using Raman spectroscopy (Conference Presentation)

Benjamin Lochocki, Tjado H. J. Morrema, Jurre den Haan, Freek Ariese, Femke H. Bouwman, Jeroen J. Hoozemans, Johannes F. de Boer

Author Affiliations +

Proceedings Volume 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019; 108901N (2019) https://doi.org/10.1117/12.2509297
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Dementia is one of the main death leading causes worldwide and Alzheimer’s disease (AD) is its most common form. In postmortem examinations of AD brain tissue, extracellular deposits of proteins are observed, known as amyloid-beta (Aß) plaques. Aß plaques are characterized by their occurrence of beta-sheets and are, beside tau tangles, biological hallmarks in the postmortem diagnosis of AD. Little research on the detectability of Aß deposits in brain tissue using Raman spectroscopy has been published. Here, we examined formalin fixed, paraffin embedded tissue slices of AD and healthy control cases. The slices have been spectrally raster imaged with a step size smaller the size of a plaque using a commercial Raman spectroscope with a NIR laser source to obtain a hyperspectral map of the size of 0.5mm2. Specific band intensities including, among others, protein and lipid components were analyzed and afterward compared to the healthy control cases to study spectral differences. Further, Aß deposit locations could be precisely matched to the obtained spectral data by staining the same Raman imaged tissue slice with Thioflavin afterward. In addition, plaques can be co-localized by using histochemical stained adjacent tissue slices. In conclusion, we present new insights on spectral changes in the Raman fingerprint region of 950 to 1800cm-1 when analyzing the molecular composition of AD brain tissue.

Conference Presentation

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Benjamin Lochocki, Tjado H. J. Morrema, Jurre den Haan, Freek Ariese, Femke H. Bouwman, Jeroen J. Hoozemans, and Johannes F. de Boer "Imaging of Aβ-deposits in ex-vivo Alzheimer's disease brain tissue using Raman spectroscopy (Conference Presentation)", Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 108901N (4 March 2019); https://doi.org/10.1117/12.2509297

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KEYWORDS

Raman spectroscopy

Brain

Imaging spectroscopy

Alzheimer's disease

Tissue optics

Tissues

Neuroimaging

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