Infrared block of Na+ and Ca2+ spikes in in crayfish neuromuscular junction

Xuedong Zhu; Jen-Wei Lin; Michelle Y. Sander

doi:10.1117/12.2508996

22 February 2019 Infrared block of Na⁺ and Ca²⁺ spikes in in crayfish neuromuscular junction

Xuedong Zhu, Jen-Wei Lin, Michelle Y. Sander

Proceedings Volume 10866, Optogenetics and Optical Manipulation 2019; 108660E (2019) https://doi.org/10.1117/12.2508996
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Pulsed infrared (IR) light in the 1.8 - 2 μm region can modulate neural activities with high spatial and temporal precision. However, the mechanisms underlying these photothermal interactions are not fully understood. Here we investigate the IR modulation of axon and muscle activities using the crayfish (Procambarus clarkii) opener neuromuscular preparation. A modulated fiber coupled laser diode (λ = 2 μm) is used to deliver pulsed light with durations between 10 – 500 ms. Twoelectrode current clamp (TECC) is performed to stimulate and monitor the neural activities. Laser-induced temperature changes are measured by an open patch pipette simultaneously with TECC. We find that IR pulses can reversibly inhibit or block axon (Na⁺ ) and muscle (Ca²⁺) spikes. In axons, single IR pulses can suppress the action potential (AP) amplitude and duration and increase the interspike interval. In addition, the rates of AP depolarization and repolarization are also modulated by IR pulses. Individual IR pulses can also block muscle fiber Ca²⁺ spikes. The IR-induced decrease in the input resistance (8.4%) can be a contributing factor for the inhibition phenomena reported here.

Citation Download Citation

Xuedong Zhu, Jen-Wei Lin, and Michelle Y. Sander "Infrared block of Na⁺ and Ca²⁺ spikes in in crayfish neuromuscular junction", Proc. SPIE 10866, Optogenetics and Optical Manipulation 2019, 108660E (22 February 2019); https://doi.org/10.1117/12.2508996

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