Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction

Irene Costantini; Miriam Menzel; Ludovico Silvestri; Nicole Schubert; Markus Axer; Katrin Amunts; Francesco S. Pavone

doi:10.1117/12.2284328

28 July 2017 Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction

Irene Costantini, Miriam Menzel, Ludovico Silvestri, Nicole Schubert, Markus Axer, Katrin Amunts, Francesco S. Pavone

Author Affiliations +

Proceedings Volume 10413, Novel Biophotonics Techniques and Applications IV; 1041306 (2017) https://doi.org/10.1117/12.2284328
Event: European Conferences on Biomedical Optics, 2017, Munich, Germany

Abstract

In this work, we employ an integrated label-free dual approach that combines Polarized Light Imaging with Two-Photon Fluorescence Microscopy to study fiber orientations of myelinated axons in fixed brain slices from different species.

Citation Download Citation

Irene Costantini, Miriam Menzel, Ludovico Silvestri, Nicole Schubert, Markus Axer, Katrin Amunts, and Francesco S. Pavone "Correlative polarized light imaging and two-photon fluorescence microscopy for 3D myelinated fibers reconstruction", Proc. SPIE 10413, Novel Biophotonics Techniques and Applications IV, 1041306 (28 July 2017); https://doi.org/10.1117/12.2284328

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