Three-photon spinning disk high-speed microscopy

Yu-Shuo Liao; Shiu-Feng Cheng; Guan-Jie Huang Huang; Jye-Chang Lee; Li-An Chu; Shang-Da Yang; Ann-Shyn Chiang; Shi-Wei Chu

doi:10.1117/12.3002157

13 March 2024 Three-photon spinning disk high-speed microscopy

Yu-Shuo Liao, Shiu-Feng Cheng, Guan-Jie Huang Huang, Jye-Chang Lee, Li-An Chu, Shang-Da Yang, Ann-Shyn Chiang, Shi-Wei Chu

Author Affiliations +

Proceedings Volume PC12853, High-Speed Biomedical Imaging and Spectroscopy IX; PC1285309 (2024) https://doi.org/10.1117/12.3002157
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

In this work, we customized a multi-photon spinning disc unit with a tunable high-power femtosecond laser to demonstrate the first three-photon spinning disk microscopy, achieving ~100Hz frame rate. We demonstrate its pioneering applications on green fluorescent beads, in vivo Drosophila brains, and fixed mouse brains. Furthermore, our findings from brain tissue imaging reveal that three-photon spinning disk imaging exhibits a significantly lower attenuation rate when compared to its two-photon spinning disk counterpart, paving the way of utilizing three-photon excitation on high-speed deep tissue imaging.

Conference Presentation

Citation Download Citation

Yu-Shuo Liao, Shiu-Feng Cheng, Guan-Jie Huang Huang, Jye-Chang Lee, Li-An Chu, Shang-Da Yang, Ann-Shyn Chiang, and Shi-Wei Chu "Three-photon spinning disk high-speed microscopy", Proc. SPIE PC12853, High-Speed Biomedical Imaging and Spectroscopy IX, PC1285309 (13 March 2024); https://doi.org/10.1117/12.3002157

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