Dual-channel two-photon fluorescence brain imaging using fiber-delivered supercontinuum source

Youbo Zhao; Smrithi Padmakumar; Mark Scimone; Gopi Maguluri; Mansoor Amiji; Nicusor Iftimia

doi:10.1117/12.2649900

17 March 2023 Dual-channel two-photon fluorescence brain imaging using fiber-delivered supercontinuum source

Youbo Zhao, Smrithi Padmakumar, Mark Scimone, Gopi Maguluri, Mansoor Amiji, Nicusor Iftimia

Proceedings Volume PC12365, Neural Imaging and Sensing 2023; PC123650E (2023) https://doi.org/10.1117/12.2649900
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We report a two-photon excitation fluorescence microscopy imager that uses a fiber-delivered fs supercontinuum (SC) generation source to achieve simultaneous dual-wavelength imaging. The SC generation is based on pumping a polarization-maintaining photonic crystal fiber with a fiber laser. The SC is then delivered to the imaging head through a broadband hollow-core fiber. Excitation wavelengths centered at 920 nm and 1180 nm were selected, which enabled simultaneous imaging of neurons transfected with GCaMP6 and administrated with muscimol-conjugated BODIPY. Imaging results of calcium dynamics of in vivo mouse brain and cultured primary neurons were collected to demonstrate the technology feasibility.

Conference Presentation

Citation Download Citation

Youbo Zhao, Smrithi Padmakumar, Mark Scimone, Gopi Maguluri, Mansoor Amiji, and Nicusor Iftimia "Dual-channel two-photon fluorescence brain imaging using fiber-delivered supercontinuum source", Proc. SPIE PC12365, Neural Imaging and Sensing 2023, PC123650E (17 March 2023); https://doi.org/10.1117/12.2649900

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