Tethered capsule endomicroscopy–guided tissue sampling of the GI tract by in vivo laser capture microdissection

Fang Hou; Rachel Smith; Tara Lignelli; Ara Bablouzian; Shwaathi Deenadayalan; Tianxia Li; Adam Bass; Guillermo Tearney

doi:10.1117/12.2650965

17 March 2023 Tethered capsule endomicroscopy–guided tissue sampling of the GI tract by in vivo laser capture microdissection

Fang Hou, Rachel Smith, Tara Lignelli, Ara Bablouzian, Shwaathi Deenadayalan, Tianxia Li, Adam Bass, Guillermo Tearney

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Proceedings Volume PC12356, Endoscopic Microscopy XVIII; PC123560J (2023) https://doi.org/10.1117/12.2650965
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

To address the need for less invasive and more accurate upper gastrointestinal biopsy, we have developed a swallowable optical-coherence tomography (OCT) tethered capsule endomicroscopy (TCE) device with image-targeted biopsy capabilities. The laser-captured biopsy can be used for histopathology and genetic analysis that could potentially improve the accuracy and reduce the cost of GI disease surveillance. Ex-vivo and in-vivo experiments on swine were conducted to optimize laser parameters, coating thickness, DNA isolation protocol, and histology of IVLCM samples. Results show that >200 ng of dsDNA can be isolated from the captured sample, which is sufficient for genetic analysis.

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Fang Hou, Rachel Smith, Tara Lignelli, Ara Bablouzian, Shwaathi Deenadayalan, Tianxia Li, Adam Bass, and Guillermo Tearney "Tethered capsule endomicroscopy–guided tissue sampling of the GI tract by in vivo laser capture microdissection", Proc. SPIE PC12356, Endoscopic Microscopy XVIII, PC123560J (17 March 2023); https://doi.org/10.1117/12.2650965

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KEYWORDS

In vivo imaging

Laser tissue interaction

Biopsy

Coating

Optical coherence tomography

Imaging systems

Tissues

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