Real-time direct observation of the formation of insulin spherulites by a new super-resolution microscopy

Min Zhang; Henrik Pinholt; Xin Zhou; Vito Foderà; Nikos Hatzakis

doi:10.1117/12.2621210

30 May 2022 Real-time direct observation of the formation of insulin spherulites by a new super-resolution microscopy

Min Zhang, Henrik Pinholt, Xin Zhou, Vito Foderà, Nikos Hatzakis

Proceedings Volume PC12136, Unconventional Optical Imaging III; PC1213613 (2022) https://doi.org/10.1117/12.2621210
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

The self-assembly of native structural proteins into fibrils or amyloid spherulite-like structures is associated with a spectrum of neurodegenerative diseases. we have a new super-resolution method (REPLOM), to observe directly and in real-time the formation of insulin spherulites and quantify the existence and abundance of diverse morphologies as well as their heterogeneous growth kinetics. Our results surprisingly revealed insulin spherulite growth is not exclusively isotropic, but it may also occur anisotropically. Combined with Machine learning we associated growth rates to specific morphological transitions and provided energy barriers and the energy landscape for each aggregation morphology.

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Min Zhang, Henrik Pinholt, Xin Zhou, Vito Foderà, and Nikos Hatzakis "Real-time direct observation of the formation of insulin spherulites by a new super-resolution microscopy", Proc. SPIE PC12136, Unconventional Optical Imaging III, PC1213613 (30 May 2022); https://doi.org/10.1117/12.2621210

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KEYWORDS

Super resolution microscopy

Proteins

Microscopy

Alzheimer's disease

Machine learning

Spectroscopy

Spherical lenses

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