Cryogenic super-resolution fluorescence correlated with cryogenic electron tomography: combining specific labelling and high resolution context

Peter Dahlberg

doi:10.1117/12.2610478

2 March 2022 Cryogenic super-resolution fluorescence correlated with cryogenic electron tomography: combining specific labelling and high resolution context

Peter Dahlberg

Author Affiliations +

Proceedings Volume PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV; PC119670A (2022) https://doi.org/10.1117/12.2610478
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Identifying biomolecules of interest in cryogenic electron tomography (CET) reconstructions is made challenging by the lack of non-perturbative and specific labeling methods compatible with CET. Combining fluorescence and CET is a promising approach to overcome this labeling limitation. However, diffraction-limited fluorescence data has insufficient resolution to provide clear labeling in the crowded cellular environment. Super-resolution fluorescence techniques achieve resolution on the tens of nanometers scale, making them compatible with the length scales of interest in labeling biomolecules in CET. I will discuss the development of a cryogenic single-molecule based super-resolution imaging approach that achieves an average localization precision of less than ten nanometers and is compatible with the latest CET methods.

Conference Presentation

Citation Download Citation

Peter Dahlberg "Cryogenic super-resolution fluorescence correlated with cryogenic electron tomography: combining specific labelling and high resolution context", Proc. SPIE PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV, PC119670A (2 March 2022); https://doi.org/10.1117/12.2610478

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