Pioneers in Neurophotonics: Special Section Honoring Professor Amiram Grinvald

Optical imaging of the rat brain suggests a previously missing link between top-down and bottom-up nervous system function

[+] Author Affiliations
Susan A. Greenfield, Giovanni Ferrati

Neuro-Bio Ltd., Building F5, Culham Science Centre, Abingdon, United Kingdom

Antoine-Scott Badin

Neuro-Bio Ltd., Building F5, Culham Science Centre, Abingdon, United Kingdom

University of Oxford, Department of Physiology, Anatomy & Genetics, Oxford, United Kingdom

Ian M. Devonshire

Nottingham University Medical School, Queen’s Medical Centre, Nottingham, United Kingdom

Neurophoton. 4(3), 031213 (May 24, 2017). doi:10.1117/1.NPh.4.3.031213
History: Received January 12, 2017; Accepted April 26, 2017
Text Size: A A A

Abstract.  Optical imaging with voltage-sensitive dyes enables the visualization of extensive yet highly transient coalitions of neurons (assemblies) operating throughout the brain on a subsecond time scale. We suggest that operating at the mesoscale level of brain organization, neuronal assemblies may provide a functional link between “bottom-up” cellular mechanisms and “top-down” cognitive ones within anatomically defined regions. We demonstrate in ex vivo rat brain slices how varying spatiotemporal dynamics of assemblies reveal differences not previously appreciated between: different stages of development in cortical versus subcortical brain areas, different sensory modalities (hearing versus vision), different classes of psychoactive drugs (anesthetics versus analgesics), different effects of anesthesia linked to hyperbaric conditions and, in vivo, depths of anesthesia. The strategy of voltage-sensitive dye imaging is therefore as powerful as it is versatile and as such can now be applied to the evaluation of neurochemical signaling systems and the screening of related new drugs, as well as to mathematical modeling and, eventually, even theories of consciousness.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Susan A. Greenfield ; Antoine-Scott Badin ; Giovanni Ferrati and Ian M. Devonshire
"Optical imaging of the rat brain suggests a previously missing link between top-down and bottom-up nervous system function", Neurophoton. 4(3), 031213 (May 24, 2017). ; http://dx.doi.org/10.1117/1.NPh.4.3.031213


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.