Pioneers in Neurophotonics: Special Section Honoring Professor Amiram Grinvald

Imaging membrane potential changes from dendritic spines using computer-generated holography

[+] Author Affiliations
Dimitrii Tanese, Valeria Zampini, Vincent De Sars, Valentina Emiliani

Paris Descartes University, Neurophotonics Laboratory, CNRS UMR8250, Paris, France

Ju-Yun Weng, Dejan Zecevic

Yale University School of Medicine, Department of Cellular and Molecular Physiology, New Haven, Connecticut, United States

Marco Canepari

Université Grenoble Alpes and CNRS, Laboratory for Interdisciplinary Physics, UMR 5588, Saint Martin d’Hères, France

Laboratories of Excellence, Ion Channel Science and Therapeutics, France

Institut National de la Santé et Recherche Médicale, Grenoble, France

Balazs Rozsa

Institute of Experimental Medicine of the Hungarian Academy of Sciences, Budapest, Hungary

Neurophoton. 4(3), 031211 (May 12, 2017). doi:10.1117/1.NPh.4.3.031211
History: Received February 28, 2017; Accepted April 24, 2017
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Abstract.  Electrical properties of neuronal processes are extraordinarily complex, dynamic, and, in the general case, impossible to predict in the absence of detailed measurements. To obtain such a measurement one would, ideally, like to be able to monitor electrical subthreshold events as they travel from synapses on distal dendrites and summate at particular locations to initiate action potentials. It is now possible to carry out these measurements at the scale of individual dendritic spines using voltage imaging. In these measurements, the voltage-sensitive probes can be thought of as transmembrane voltmeters with a linear scale, which directly monitor electrical signals. Grinvald et al. were important early contributors to the methodology of voltage imaging, and they pioneered some of its significant results. We combined voltage imaging and glutamate uncaging using computer-generated holography. The results demonstrated that patterned illumination, by reducing the surface area of illuminated membrane, reduces photodynamic damage. Additionally, region-specific illumination practically eliminated the contamination of optical signals from individual spines by the scattered light from the parent dendrite. Finally, patterned illumination allowed one-photon uncaging of glutamate on multiple spines to be carried out in parallel with voltage imaging from the parent dendrite and neighboring spines.

Figures in this Article
© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Dimitrii Tanese ; Ju-Yun Weng ; Valeria Zampini ; Vincent De Sars ; Marco Canepari, et al.
"Imaging membrane potential changes from dendritic spines using computer-generated holography", Neurophoton. 4(3), 031211 (May 12, 2017). ; http://dx.doi.org/10.1117/1.NPh.4.3.031211


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