Research Papers

Ryanodine and IP3 receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation

[+] Author Affiliations
Gleb P. Tolstykh

General Dynamics Information Technology, JBSA Fort Sam Houston, San Antonio, Texas, United States

Cory A. Olsovsky

Texas A&M University, Department of Biomedical Engineering, College Station, Texas, United States

Bennett L. Ibey

Air Force Research Laboratory, 711th Human Performance Wing, Airman Systems Directorate, Bioeffects Division, Radio Frequency Bioeffects Branch, JBSA Fort Sam Houston, San Antonio, Texas, United States

Hope T. Beier

Air Force Research Laboratory, 711th Human Performance Wing, Airman System Directorate, Bioeffects Division, Optical Radiation Bioeffects Branch, JBSA Fort Sam Houston, San Antonio, Texas, United States

Neurophoton. 4(2), 025001 (Apr 05, 2017). doi:10.1117/1.NPh.4.2.025001
History: Received December 1, 2016; Accepted March 20, 2017
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Abstract.  Pulsed infrared (IR) laser energy has been shown to modulate neurological activity through both stimulation and inhibition of action potentials. While the mechanism(s) behind this phenomenon is (are) not completely understood, certain hypotheses suggest that the rise in temperature from IR exposure could activate temperature- or pressure-sensitive ion channels or create pores in the cellular outer membrane, allowing an influx of typically plasma-membrane-impermeant ions. Studies using fluorescent intensity-based calcium ion (Ca2+) sensitive dyes show changes in Ca2+ levels after various IR stimulation parameters, which suggests that Ca2+ may originate from the external solution. However, activation of intracellular signaling pathways has also been demonstrated, indicating a more complex mechanism of increasing intracellular Ca2+ concentration. We quantified the Ca2+ mobilization in terms of influx from the external solution and efflux from intracellular organelles using Fura-2 and a high-speed ratiometric imaging system that rapidly alternates the dye excitation wavelengths. Using nonexcitable Chinese hamster ovarian (CHO-hM1) cells and neuroblastoma-glioma (NG108) cells, we demonstrate that intracellular IP3 receptors play an important role in the IR-induced Ca2+, with the Ca2+ response augmented by ryanodine receptors in excitable cells.

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© 2017 Society of Photo-Optical Instrumentation Engineers

Citation

Gleb P. Tolstykh ; Cory A. Olsovsky ; Bennett L. Ibey and Hope T. Beier
"Ryanodine and IP3 receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation", Neurophoton. 4(2), 025001 (Apr 05, 2017). ; http://dx.doi.org/10.1117/1.NPh.4.2.025001


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