Research Papers

Two-photon microscopy measurement of cerebral metabolic rate of oxygen using periarteriolar oxygen concentration gradients

[+] Author Affiliations
Sava Sakadžić, Mohammad A. Yaseen, Rajeshwer Jaswal, David A. Boas

Massachusetts General Hospital and Harvard Medical School, Optics Division, MGH/HMS/MIT Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, 149 13th Street, Charlestown, Massachusetts 02129, United States

Emmanuel Roussakis, Sergei A. Vinogradov

University of Pennsylvania, Departments of Biochemistry and Biophysics and Chemistry, 422 Curie Boulevard, Philadelphia, Pennsylvania 19104, United States

Anders M. Dale

University of California San Diego, Department of Neurosciences, 9500 Gilman Drive, La Jolla, California 92093, United States

University of California San Diego, Department of Radiology, 9500 Gilman Drive, La Jolla, California 92093, United States

Richard B. Buxton

University of California San Diego, Department of Radiology, 9500 Gilman Drive, La Jolla, California 92093, United States

Anna Devor

Massachusetts General Hospital and Harvard Medical School, Optics Division, MGH/HMS/MIT Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, 149 13th Street, Charlestown, Massachusetts 02129, United States

University of California San Diego, Department of Neurosciences, 9500 Gilman Drive, La Jolla, California 92093, United States

University of California San Diego, Department of Radiology, 9500 Gilman Drive, La Jolla, California 92093, United States

Neurophoton. 3(4), 045005 (Oct 17, 2016). doi:10.1117/1.NPh.3.4.045005
History: Received April 23, 2016; Accepted September 16, 2016
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Abstract.  The cerebral metabolic rate of oxygen (CMRO2) is an essential parameter for evaluating brain function and pathophysiology. However, the currently available approaches for quantifying CMRO2 rely on complex multimodal imaging and mathematical modeling. Here, we introduce a method that allows estimation of CMRO2 based on a single measurement modality—two-photon imaging of the partial pressure of oxygen (PO2) in cortical tissue. We employed two-photon phosphorescence lifetime microscopy (2PLM) and the oxygen-sensitive nanoprobe PtP-C343 to map the tissue PO2 distribution around cortical penetrating arterioles. CMRO2 is subsequently estimated by fitting the changes of tissue PO2 around arterioles with the Krogh cylinder model of oxygen diffusion. We measured the baseline CMRO2 in anesthetized rats and modulated tissue PO2 levels by manipulating the depth of anesthesia. This method provides CMRO2 measurements localized within 200  μm and it may provide oxygen consumption measurements in individual cortical layers or within confined cortical regions, such as in ischemic penumbra and the foci of functional activation.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Sava Sakadžić ; Mohammad A. Yaseen ; Rajeshwer Jaswal ; Emmanuel Roussakis ; Anders M. Dale, et al.
"Two-photon microscopy measurement of cerebral metabolic rate of oxygen using periarteriolar oxygen concentration gradients", Neurophoton. 3(4), 045005 (Oct 17, 2016). ; http://dx.doi.org/10.1117/1.NPh.3.4.045005


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