Special Section on Brain Mapping and Therapeutics

Visualizing and mapping the cerebellum with serial optical coherence scanner

[+] Author Affiliations
Chao J. Liu, Kristen E. Williams, Taner Akkin

University of Minnesota, Department of Biomedical Engineering, 312 Church Street S.E., Minneapolis, Minnesota 55455, United States

Harry T. Orr

University of Minnesota, Institute of Translational Neuroscience, 2101 6th Street S.E., Minneapolis, Minnesota 55455, United States

University of Minnesota, Department of Laboratory Medicine and Pathology, 420 Delaware Street S.E., Minneapolis, Minnesota 55455, United States

Neurophoton. 4(1), 011006 (Sep 30, 2016). doi:10.1117/1.NPh.4.1.011006
History: Received July 1, 2016; Accepted September 12, 2016
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Abstract.  We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography for ex vivo imaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.

Figures in this Article
© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Chao J. Liu ; Kristen E. Williams ; Harry T. Orr and Taner Akkin
"Visualizing and mapping the cerebellum with serial optical coherence scanner", Neurophoton. 4(1), 011006 (Sep 30, 2016). ; http://dx.doi.org/10.1117/1.NPh.4.1.011006


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