Special Section on Causal Control of Biological Systems with Light

All-optical bidirectional neural interfacing using hybrid multiphoton holographic optogenetic stimulation

[+] Author Affiliations
Shir Paluch-Siegler, Tom Mayblum, Inbar Brosh, Shy Shoham

Technion—Israel Institute of Technology, Faculty of Biomedical Engineering, Technion City, Haifa 3200000, Israel

Hod Dana

Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia 20147, United States

Inna Gefen

Ruppin Academic Center, School of Engineering, Medical Engineering, Emeq Hefer 4025000, Israel

Neurophoton. 2(3), 031208 (Jul 23, 2015). doi:10.1117/1.NPh.2.3.031208
History: Received January 20, 2015; Accepted June 15, 2015
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Abstract.  Our understanding of neural information processing could potentially be advanced by combining flexible three-dimensional (3-D) neuroimaging and stimulation. Recent developments in optogenetics suggest that neurophotonic approaches are in principle highly suited for noncontact stimulation of network activity patterns. In particular, two-photon holographic optical neural stimulation (2P-HONS) has emerged as a leading approach for multisite 3-D excitation, and combining it with temporal focusing (TF) further enables axially confined yet spatially extended light patterns. Here, we study key steps toward bidirectional cell-targeted 3-D interfacing by introducing and testing a hybrid new 2P-TF-HONS stimulation path for accurate parallel optogenetic excitation into a recently developed hybrid multiphoton 3-D imaging system. The system is shown to allow targeted all-optical probing of in vitro cortical networks expressing channelrhodopsin-2 using a regeneratively amplified femtosecond laser source tuned to 905 nm. These developments further advance a prospective new tool for studying and achieving distributed control over 3-D neuronal circuits both in vitro and in vivo.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Shir Paluch-Siegler ; Tom Mayblum ; Hod Dana ; Inbar Brosh ; Inna Gefen, et al.
"All-optical bidirectional neural interfacing using hybrid multiphoton holographic optogenetic stimulation", Neurophoton. 2(3), 031208 (Jul 23, 2015). ; http://dx.doi.org/10.1117/1.NPh.2.3.031208


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