Research Papers

Optical coherence tomography for cross-sectional imaging of neural activity

[+] Author Affiliations
Yi-Jou Yeh

University of Minnesota, Department of Electrical and Computer Engineering, Minneapolis, Minnesota 55455, United States

University of Minnesota, Department of Biomedical Engineering, 312 Church Street S.E., Minneapolis, Minnesota 55455, United States

Adam J. Black, Taner Akkin

University of Minnesota, Department of Biomedical Engineering, 312 Church Street S.E., Minneapolis, Minnesota 55455, United States

David Landowne

University of Miami, Department of Physiology and Biophysics, Miami, Florida 33101, United States

Neurophoton. 2(3), 035001 (Jul 21, 2015). doi:10.1117/1.NPh.2.3.035001
History: Received October 17, 2014; Accepted June 18, 2015
Text Size: A A A

Abstract.  We report a functional optical coherence tomography cross-sectional scanner to detect neural activity using unmyelinated nerves dissected from squid. The nerves, unstained or stained with a voltage-sensitive dye, were imaged in a nerve chamber. Transient phase changes from backscattered light were detected during action potential propagation. The results show that the scanner can provide high spatiotemporal resolution cross-sectional images of neural activity (15μs/A-line; 0.25ms/B-scan; 8.5×5.5μm2 in xz). The advantage of this method compared to monitoring a single depth profile z is a dramatic increase in the number of available sites that can be measured in two spatial dimensions xz with lateral scanning; therefore, the study demonstrates that two-dimensional monitoring of small-scale functional activity would also be feasible.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Yi-Jou Yeh ; Adam J. Black ; David Landowne and Taner Akkin
"Optical coherence tomography for cross-sectional imaging of neural activity", Neurophoton. 2(3), 035001 (Jul 21, 2015). ; http://dx.doi.org/10.1117/1.NPh.2.3.035001


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.