Special Section on Causal Control of Biological Systems with Light

Modulating dopamine release by optogenetics in transgenic mice reveals terminal dopaminergic dynamics

[+] Author Affiliations
Yao Lu

Brown University, School of Engineering, Providence, Rhode Island 02912, United States

Brown University, Department of Chemistry, Providence, Rhode Island 02912, United States

Nicolette Driscoll, Ilker Ozden, Zeyang Yu

Brown University, School of Engineering, Providence, Rhode Island 02912, United States

Arto V. Nurmikko

Brown University, School of Engineering, Providence, Rhode Island 02912, United States

Brown University, Institute of Brain Science, Providence, Rhode Island 02912, United States

Neurophoton. 2(3), 031207 (Jul 09, 2015). doi:10.1117/1.NPh.2.3.031207
History: Received February 17, 2015; Accepted June 15, 2015
Text Size: A A A

Abstract.  Dopamine (DA) release and uptake dynamics in the nucleus accumbens (NAc) have important implications for neurological diseases and mammalian animal behaviors. We demonstrate here the use of cell-type-specific optogenetic targeting in conjunction with fast-scan cyclic voltammetry applied to brain slices prepared from specifically tailored transgenic mice, which conditionally express channelrhodopsin-2 (ChR2) through dopamine transporter (DAT)-Cre. Terminal dopaminergic dynamics and the direct manipulation of induced DA release level by controlling light intensity, pulse width, and the shape of stimulation waveforms were studied. Effective cell terminal-targeting optogenetic induction of DA release at physiological levels in NAc is demonstrated and discussed. It was found that delivering more light energy by increasing stimulation intensity and length is not the only way to control DA release; the temporal shape of the stimulus waveform at light onset is also critically related to induced DA concentrations. In addition, DA uptake dynamics as well as the recovery of the presynaptic releasable DA pool are studied and modeled. More broadly, our experimental findings provide important further evidence for effectively applying optogenetics to induce neurotransmitter release in the behaviorally relevant region of the brain in a highly cell-type selective context.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Yao Lu ; Nicolette Driscoll ; Ilker Ozden ; Zeyang Yu and Arto V. Nurmikko
"Modulating dopamine release by optogenetics in transgenic mice reveals terminal dopaminergic dynamics", Neurophoton. 2(3), 031207 (Jul 09, 2015). ; http://dx.doi.org/10.1117/1.NPh.2.3.031207


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.