Special Section on Causal Control of Biological Systems with Light

Red fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications

[+] Author Affiliations
Yi Shen, Tiffany Lai, Robert E. Campbell

University of Alberta, Department of Chemistry, Edmonton, Alberta T6G 2G2, Canada

Neurophoton. 2(3), 031203 (Jun 19, 2015). doi:10.1117/1.NPh.2.3.031203
History: Received December 2, 2014; Accepted May 19, 2015
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Abstract.  The inherent advantages of red-shifted fluorescent proteins and fluorescent protein-based biosensors for the study of signaling processes in neurons and other tissues have motivated the development of a plethora of new tools. Relative to green fluorescent proteins (GFPs) and other blue-shifted alternatives, red fluorescent proteins (RFPs) provide the inherent advantages of lower phototoxicity, lower autofluorescence, and deeper tissue penetration associated with longer wavelength excitation light. All other factors being the same, the multiple benefits of using RFPs make these tools seemingly ideal candidates for use in neurons and, ultimately, the brain. However, for many applications, the practical utility of RFPs still falls short of the preferred GFPs. We present an overview of RFPs and RFP-based biosensors, with an emphasis on their reported applications in neuroscience.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Yi Shen ; Tiffany Lai and Robert E. Campbell
"Red fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications", Neurophoton. 2(3), 031203 (Jun 19, 2015). ; http://dx.doi.org/10.1117/1.NPh.2.3.031203


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