Research Papers

Multimodal reconstruction of microvascular-flow distributions using combined two-photon microscopy and Doppler optical coherence tomography

[+] Author Affiliations
Louis Gagnon

Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, United States

Harvard-MIT Division of Health Sciences and Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States

Ecole Polytechnique de Montreal, Department of Electrical Engineering, 2900 Boulevard Edouard-Montpetit, Montreal, Quebec H3T 1J4, Canada

Sava Sakadžić, Emiri T. Mandeville, Qianqian Fang, Mohammad A. Yaseen

Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, United States

Fréderic Lesage

Ecole Polytechnique de Montreal, Department of Electrical Engineering, 2900 Boulevard Edouard-Montpetit, Montreal, Quebec H3T 1J4, Canada

David A. Boas

Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Building 149 13th Street, Charlestown, Massachusetts 02129, United States

Harvard-MIT Division of Health Sciences and Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States

Neurophoton. 2(1), 015008 (Mar 12, 2015). doi:10.1117/1.NPh.2.1.015008
History: Received October 7, 2014; Accepted February 20, 2015
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Abstract.  Computing microvascular cerebral blood flow (μCBF) in real cortical angiograms is challenging. Here, we investigated whether the use of Doppler optical coherence tomography (DOCT) flow measurements in individual vessel segments can help in reconstructing μCBF across the entire vasculature of a truncated cortical angiogram. A μCBF computational framework integrating DOCT measurements is presented. Simulations performed on a synthetic angiogram showed that the addition of DOCT measurements, especially close to large inflowing or outflowing vessels, reduces the impact of pressure boundary conditions and estimated vessel resistances resulting in a more accurate reconstruction of μCBF. Our technique was then applied to reconstruct microvascular flow distributions in the mouse cortex down to 660μm by combining two-photon laser scanning microscopy angiography with DOCT.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Louis Gagnon ; Sava Sakadžić ; Fréderic Lesage ; Emiri T. Mandeville ; Qianqian Fang, et al.
"Multimodal reconstruction of microvascular-flow distributions using combined two-photon microscopy and Doppler optical coherence tomography", Neurophoton. 2(1), 015008 (Mar 12, 2015). ; http://dx.doi.org/10.1117/1.NPh.2.1.015008


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