Research Papers

Optical coherence tomography visualizes neurons in human entorhinal cortex

[+] Author Affiliations
Caroline Magnain, Jean C. Augustinack, Ender Konukoglu, Sava Sakadžić, Ani Varjabedian, Nathalie Garcia, Van J. Wedeen, David A. Boas

Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital/Harvard Medical School, Department of Radiology, 149 Thirteen Street, Charlestown, Massachusetts 02129, United States

Matthew P. Frosch

Massachusetts General Hospital, Pathology Service, C.S. Kubik Laboratory for Neuropathology, Warren Building 225, 55 Fruit Street, Boston, Massachusetts 02115, United States

Bruce Fischl

Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital/Harvard Medical School, Department of Radiology, 149 Thirteen Street, Charlestown, Massachusetts 02129, United States

MIT, Computer Science and AI Laboratory, the Stata Center, Building 32, 32 Vassar Street, Cambridge, Massachusetts 02139, United States

Neurophoton. 2(1), 015004 (Feb 09, 2015). doi:10.1117/1.NPh.2.1.015004
History: Received September 12, 2014; Accepted January 12, 2015
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Abstract.  The cytoarchitecture of the human brain is of great interest in diverse fields: neuroanatomy, neurology, neuroscience, and neuropathology. Traditional histology is a method that has been historically used to assess cell and fiber content in the ex vivo human brain. However, this technique suffers from significant distortions. We used a previously demonstrated optical coherence microscopy technique to image individual neurons in several square millimeters of en-face tissue blocks from layer II of the human entorhinal cortex, over 50μm in depth. The same slices were then sectioned and stained for Nissl substance. We registered the optical coherence tomography (OCT) images with the corresponding Nissl stained slices using a nonlinear transformation. The neurons were then segmented in both images and we quantified the overlap. We show that OCT images contain information about neurons that is comparable to what can be obtained from Nissl staining, and thus can be used to assess the cytoarchitecture of the ex vivo human brain with minimal distortion. With the future integration of a vibratome into the OCT imaging rig, this technique can be scaled up to obtain undistorted volumetric data of centimeter cube tissue blocks in the near term, and entire human hemispheres in the future.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Caroline Magnain ; Jean C. Augustinack ; Ender Konukoglu ; Matthew P. Frosch ; Sava Sakadžić, et al.
"Optical coherence tomography visualizes neurons in human entorhinal cortex", Neurophoton. 2(1), 015004 (Feb 09, 2015). ; http://dx.doi.org/10.1117/1.NPh.2.1.015004


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