Special Section on the BRAIN Initiative

Characterization of the BAC Id3-enhanced green fluorescent protein transgenic mouse line for in vivo imaging of astrocytes

[+] Author Affiliations
Cassandra Lamantia

University of Rochester, Department of Neurobiology and Anatomy, Rochester, New York 14642, United States

Marie-Eve Tremblay

University of Rochester, Department of Neurobiology and Anatomy, Rochester, New York 14642, United States

Ania Majewska

University of Rochester, Department of Neurobiology and Anatomy, Rochester, New York 14642, United States

Neurophoton. 1(1), 011014 (Sep 12, 2014). doi:10.1117/1.NPh.1.1.011014
History: Received March 24, 2014; Revised August 6, 2014; Accepted September 3, 2014
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Abstract.  Astrocytes are highly ramified glial cells with critical roles in brain physiology and pathology. Recently, breakthroughs in imaging technology have expanded our understanding of astrocyte function in vivo. The in vivo study of astrocytic dynamics, however, is limited by the tools available to label astrocytes and their processes. Here, we characterize the bacterial artificial chromosome transgenic Id3-EGFP knock-in mouse to establish its usefulness for in vivo imaging of astrocyte processes. Using fixed brain sections, we observed enhanced green fluorescent protein expression in astrocytes and blood vessel walls throughout the brain, although the extent and cell type specificity of expression depended on the brain area and developmental age. Using in vivo two-photon imaging, we visualized astrocytes in cortical layers 1–3 in both thin skull and window preparations. In adult animals, astrocytic cell bodies and fine processes could be followed over many hours. Our results suggest that Id3 mice could be used for in vivo imaging of astrocytes and blood vessels in development and adulthood.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Cassandra Lamantia ; Marie-Eve Tremblay and Ania Majewska
"Characterization of the BAC Id3-enhanced green fluorescent protein transgenic mouse line for in vivo imaging of astrocytes", Neurophoton. 1(1), 011014 (Sep 12, 2014). ; http://dx.doi.org/10.1117/1.NPh.1.1.011014


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