Research Papers

Nondestructive evaluation of progressive neuronal changes in organotypic rat hippocampal slice cultures using ultrahigh-resolution optical coherence microscopy

[+] Author Affiliations
Fengqiang Li

Lehigh University, Department of Electrical and Computer Engineering, 19 Memorial Drive West, Bethlehem, Pennsylvania 18015, United States

Lehigh University, Center for Photonics and Nanoelectronics, 7 ASA Drive, Bethlehem, Pennsylvania 18015, United States

Yu Song

Lehigh University, Bioengineering Program, 111 Research Drive, Bethlehem, Pennsylvania 18015, United States

Alexandra Dryer

Lehigh University, Department of Electrical and Computer Engineering, 19 Memorial Drive West, Bethlehem, Pennsylvania 18015, United States

William Cogguillo

Lehigh University, Bioengineering Program, 111 Research Drive, Bethlehem, Pennsylvania 18015, United States

Yevgeny Berdichevsky

Lehigh University, Department of Electrical and Computer Engineering, 19 Memorial Drive West, Bethlehem, Pennsylvania 18015, United States

Lehigh University, Center for Photonics and Nanoelectronics, 7 ASA Drive, Bethlehem, Pennsylvania 18015, United States

Lehigh University, Bioengineering Program, 111 Research Drive, Bethlehem, Pennsylvania 18015, United States

Chao Zhou

Lehigh University, Department of Electrical and Computer Engineering, 19 Memorial Drive West, Bethlehem, Pennsylvania 18015, United States

Lehigh University, Center for Photonics and Nanoelectronics, 7 ASA Drive, Bethlehem, Pennsylvania 18015, United States

Lehigh University, Bioengineering Program, 111 Research Drive, Bethlehem, Pennsylvania 18015, United States

Neurophoton. 1(2), 025002 (Sep 02, 2014). doi:10.1117/1.NPh.1.2.025002
History: Received April 21, 2014; Revised July 22, 2014; Accepted July 25, 2014
Text Size: A A A

Abstract.  Three-dimensional tissue cultures have been used as effective models for studying different diseases, including epilepsy. High-throughput, nondestructive techniques are essential for rapid assessment of disease-related processes, such as progressive cell death. An ultrahigh-resolution optical coherence microscopy (UHR-OCM) system with 1.5μm axial resolution and 2.3μm transverse resolution was developed to evaluate seizure-induced neuronal injury in organotypic rat hippocampal cultures. The capability of UHR-OCM to visualize cells in neural tissue was confirmed by comparison of UHR-OCM images with confocal immunostained images of the same cultures. In order to evaluate the progression of neuronal injury, UHR-OCM images were obtained from cultures on 7, 14, 21, and 28 days in vitro (DIVs). In comparison to DIV 7, statistically significant reductions in three-dimensional cell count and culture thickness from UHR-OCM images were observed on subsequent time points. In cultures treated with kynurenic acid, significantly less reduction in cell count and culture thickness was observed compared to the control specimens. These results demonstrate the capability of UHR-OCM to perform rapid, label-free, and nondestructive evaluation of neuronal death in organotypic hippocampal cultures. UHR-OCM, in combination with three-dimensional tissue cultures, can potentially prove to be a promising tool for high-throughput screening of drugs targeting various disorders.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Fengqiang Li ; Yu Song ; Alexandra Dryer ; William Cogguillo ; Yevgeny Berdichevsky, et al.
"Nondestructive evaluation of progressive neuronal changes in organotypic rat hippocampal slice cultures using ultrahigh-resolution optical coherence microscopy", Neurophoton. 1(2), 025002 (Sep 02, 2014). ; http://dx.doi.org/10.1117/1.NPh.1.2.025002


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.